Journal: Lipids in Health and Disease

Article Title: The mechanism of apoliprotein A1 down-regulated by Hepatitis B virus

doi: 10.1186/s12944-016-0232-5

Figure Lengend Snippet: Suppression of ApoA1 expression by HBV. a and b ApoA1 mRNA and protein levels were detected by RT-PCR and Western blot in HepG2.2.15 corresponding HepG2 cell lines. c and d HepG2 cells were transfected with 2 μg pHBV1.3 plasmid or 2 μg pCDNA3.1 as control, ApoA1 mRNA and protein levels were detected at 48 h after transfection. Data are presented as the mean ± SD from three independent experiments. * p < 0.05 and ** P < 0.01 compared with mock

Article Snippet: Human hepatocellular carcinoma HepG2 and HepG2.2.15 cell lines were obtained from the ATCC (Rockville, MD), HepG2.2.15 with abilities to produce HBV virus stably is derived from HepG2 cell lines [ ].

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Transfection, Plasmid Preparation, Control

Journal: Lipids in Health and Disease

Article Title: The mechanism of apoliprotein A1 down-regulated by Hepatitis B virus

doi: 10.1186/s12944-016-0232-5

Figure Lengend Snippet: ApoA1 expression was suppressed by DNA methyltransferase inhibitor 5-aza-dC. a and b 5 CpG islands including two methylation CpG status in ApoA1 promotor were listed ( a ), the detection results of the 5 CpG islands methylation status were shown ( b ). HepG2.2.15 cells treated with 5 μM 5-aza-dC 48 h, ApoA1 mRNA and protein levels were detected by RT-PCR and Western blot respectively ( c and d ). Secretion of HBsAg and HBV particles in the supernatant were detected by ELISA and RT- PCR respectivly ( e and f ). Data are presented as the mean ± SD from three independent experiments. * p < 0.05 and ** P < 0.01 compared with mock

Article Snippet: Human hepatocellular carcinoma HepG2 and HepG2.2.15 cell lines were obtained from the ATCC (Rockville, MD), HepG2.2.15 with abilities to produce HBV virus stably is derived from HepG2 cell lines [ ].

Techniques: Expressing, Methylation, Reverse Transcription Polymerase Chain Reaction, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Lipids in Health and Disease

Article Title: The mechanism of apoliprotein A1 down-regulated by Hepatitis B virus

doi: 10.1186/s12944-016-0232-5

Figure Lengend Snippet: Decreased HBV expression with 5-aza-dC treatment via up-regulation of ApoA1 expression. a ApoA1 overexpression inversely suppressed HBV expression. HepG2 cells were cotransfected with 1 μg pApoA1 plasmid and 1 μg pHBV1.3. Sectetion of HBsAg and HBeAg were analyzed by ELISA at 48 h after transfection. b The inhibitory effect of 5-aza-dC on HBV expression was completely abolished by blocking 5-aza-dC-induced up-regulation of ApoA1 using RNAi. HepG2.2.15 cells were treated with 5 μM 5-aza-dC plus 50 μM ApoA1 siRNA or negative control, expression of HBsAg and HBeAg in the supernatant were analyzed by ELISA at 48 h. Data are presented as the mean ± SD from three independent experiments. * p < 0.05 and ** P < 0.01 compared with mock

Article Snippet: Human hepatocellular carcinoma HepG2 and HepG2.2.15 cell lines were obtained from the ATCC (Rockville, MD), HepG2.2.15 with abilities to produce HBV virus stably is derived from HepG2 cell lines [ ].

Techniques: Expressing, Over Expression, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Transfection, Blocking Assay, Negative Control

Journal: Nutrients

Article Title: Nicotinamide Adenine Dinucleotide Precursor Suppresses Hepatocellular Cancer Progression in Mice

doi: 10.3390/nu15061447

Figure Lengend Snippet: NR supplementation inhibited hematogenous multi-organ metastasis of HCC cells in mice. ( A ) Schematic of the hematogenous metastatic neoplasm model. ( B ) Trends in body weight of mice during the intervention. ( C ) Differences in weight changes between NR and control group after 37 days of intervention. Dotted line represented the line (y = 0). ( D ) The activity of HepG2-GL cells in vivo throughout the intervention, quantified by bioluminescent signals (Total Flux, p/s) of the whole mice body. Dotted line showed the bioluminescence signals on day 0. ( E ) The metastasis of HepG2-GL cells in vivo after 37 days of intervention shown by noninvasive bioluminescence imaging. ( F ) The metastasis of HepG2-GL cells in lungs after 37 days of intervention shown by bioluminescence imaging. ( G ) Quantification of the image in ( F ) with bioluminescent signals (Total Flux, p/s) of the lungs. ( H ) Lung-to-body weight ratios at the endpoint of the intervention. ( I ) Representative images of lungs dissected from mice per group. In the upper row, the arrow points to the macroscopic tumor nodule. In the lower row are scanning pictures of H&E staining sections, scale bars: 1 mm. ( J ) Representative H&E staining section of tumor metastasis to the bone and liver of mouse in the control group; scale bars: 200 μm (top row), 100 μm (bottom row). n = 7/group; Red dots: control mice; blue dots: NR-treated mice. * p < 0.05 compared to the control group. p -values were calculated using two-way RM ANOVA ( B ), unpaired t test and Welch’s correction ( C , H ), two-way RM ANOVA and Sidak’s multiple comparisons test ( D ), and Mann – Whitney test ( G ). NR, nicotinamide riboside.

Article Snippet: To establish the GFP and luciferase-labeled HepG2 cell line (HepG2-GL), HepG2 cells were transduced by ultra-purified lentivirus particles containing a plasmid encoding luciferase and eGFP (EX-hLUC-Lv201, Genecopoeia, Rockville, MD, USA) following the manufacturer’s protocol.

Techniques: Activity Assay, In Vivo, Imaging, Staining, MANN-WHITNEY

Journal: Nutrients

Article Title: Nicotinamide Adenine Dinucleotide Precursor Suppresses Hepatocellular Cancer Progression in Mice

doi: 10.3390/nu15061447

Figure Lengend Snippet: NR inhibited migration and invasion of HepG2 cells. ( A ) The cell viability of LO2 and HepG2 cells after 48-h NR treatment. ( B ) Wound-healing assay showing cell migration of HepG2 cells after 48 h. ( C ) Transwell assays showing cell migration and invasion of HepG2 cells after 24 h. n = 3; * p < 0.05, ** p < 0.01 compared to the TGF-β group. p -values were calculated using Kruskal – Wallis test and Dunn’s multiple comparisons test ( A ), one-way ANOVA and Tukey’s multiple comparisons test (( B , C )-migration), and one-way ANOVA and Tukey’s multiple comparisons test (( C )-invasion). NR, nicotinamide riboside; TGF-β, transforming growth factor-β.

Article Snippet: To establish the GFP and luciferase-labeled HepG2 cell line (HepG2-GL), HepG2 cells were transduced by ultra-purified lentivirus particles containing a plasmid encoding luciferase and eGFP (EX-hLUC-Lv201, Genecopoeia, Rockville, MD, USA) following the manufacturer’s protocol.

Techniques: Migration, Wound Healing Assay

Journal: Antioxidants

Article Title: Myrobalan Fruit Extracts Modulate Immunobiochemical Pathways In Vitro

doi: 10.3390/antiox14030350

Figure Lengend Snippet: Effects of BB3 and the fruit extracts on cell viability. HepG2 cells were treated with vehicle (EtOH) or BB3 or myrobalan extracts (12.5–200 μg/mL) for 24 ( A ), 48 ( B ), and 72 h ( C ). Mean values ± S.E.M. of four independent experiments run in duplicates (* p < 0.05 and ** p < 0.005; compared to untreated cells) are shown.

Article Snippet: The human liver cancer-derived cell line HepG2 (RRID:CVCL_0027; DSMZ, Germany) was cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco—Thermo Fisher Scientific, Vienna, Austria) supplemented with 10% ( v / v ) heat-inactivated FCS.

Techniques:

Journal: Antioxidants

Article Title: Myrobalan Fruit Extracts Modulate Immunobiochemical Pathways In Vitro

doi: 10.3390/antiox14030350

Figure Lengend Snippet: Measurement of intracellular ROS. Inhibition of peroxyl-radical (AAPH)-induced formation of ROS in HepG2 cells pretreated with increasing concentrations of BB3 or the myrobalan extracts (12.5–200 μg/mL). The mean percentages of DCF fluorescence, as a measure of ROS formation, are shown in relation to the AAPH-treated EtOH vehicle control (set to 100%). Mean values ± S.E.M. of three independent experiments run in quadruplicates (** p < 0.01; compared to AAPH-treated cells) are shown.

Article Snippet: The human liver cancer-derived cell line HepG2 (RRID:CVCL_0027; DSMZ, Germany) was cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco—Thermo Fisher Scientific, Vienna, Austria) supplemented with 10% ( v / v ) heat-inactivated FCS.

Techniques: Inhibition, Fluorescence, Control

Journal: Antioxidants

Article Title: Myrobalan Fruit Extracts Modulate Immunobiochemical Pathways In Vitro

doi: 10.3390/antiox14030350

Figure Lengend Snippet: Influence of plant extracts on ß-lactamase activity, which was taken as a measure for ARE-dependent gene expression, in CellSensor ® ARE-bla HepG2 cells (expressed as n-fold to the corresponding control solvent). Shown are the mean values ± S.E.M. of three independent experiments with four parallels per concentration. (* p < 0.05 and ** p < 0.01; compared to vehicle-treated control cells).

Article Snippet: The human liver cancer-derived cell line HepG2 (RRID:CVCL_0027; DSMZ, Germany) was cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco—Thermo Fisher Scientific, Vienna, Austria) supplemented with 10% ( v / v ) heat-inactivated FCS.

Techniques: Activity Assay, Gene Expression, Control, Solvent, Concentration Assay

Journal: Journal of Natural Science, Biology, and Medicine

Article Title: Influence of limonin on Wnt signalling molecule in HepG2 cell lines

doi: 10.4103/0976-9668.107276

Figure Lengend Snippet: Effect of Limonin on HepG2 cells viability - MTT assay Each Bar represents mean ±SD of six observations, a -Control Vs DMSO, 20, 40, 60, 80 and 100 μM 24 h, b -Control Vs DMSO, 20, 40, 60, 80 and 100 μM for 48 h

Article Snippet: Human liver-derived hepatoma G2 (HepG2) cells was obtained from National Centre for Cell Science (NCCS), Pune, India.

Techniques: MTT Assay

Journal: Journal of Natural Science, Biology, and Medicine

Article Title: Influence of limonin on Wnt signalling molecule in HepG2 cell lines

doi: 10.4103/0976-9668.107276

Figure Lengend Snippet: Viability and lactate dehydrogenase leakage in control and limonin treated HepG2 cells after 24 hours of exposure, Each bar represents mean SD of six observations, a -Group I Vs Group II & Group III, b -Group II Vs Group III

Article Snippet: Human liver-derived hepatoma G2 (HepG2) cells was obtained from National Centre for Cell Science (NCCS), Pune, India.

Techniques:

Journal: Journal of Natural Science, Biology, and Medicine

Article Title: Influence of limonin on Wnt signalling molecule in HepG2 cell lines

doi: 10.4103/0976-9668.107276

Figure Lengend Snippet: Levels of GSH in control and limonin treated HepG2 cells, Each bar represents mean SD of six observations, a -Group I Vs Group II & Group III, b -Group II Vs Group III

Article Snippet: Human liver-derived hepatoma G2 (HepG2) cells was obtained from National Centre for Cell Science (NCCS), Pune, India.

Techniques:

Journal: Journal of Natural Science, Biology, and Medicine

Article Title: Influence of limonin on Wnt signalling molecule in HepG2 cell lines

doi: 10.4103/0976-9668.107276

Figure Lengend Snippet: Microscopic image of control and limonin treated HepG2 cells

Article Snippet: Human liver-derived hepatoma G2 (HepG2) cells was obtained from National Centre for Cell Science (NCCS), Pune, India.

Techniques:

Journal: Journal of Natural Science, Biology, and Medicine

Article Title: Influence of limonin on Wnt signalling molecule in HepG2 cell lines

doi: 10.4103/0976-9668.107276

Figure Lengend Snippet: Differential mRNA expression of p53 in HepG2 cells

Article Snippet: Human liver-derived hepatoma G2 (HepG2) cells was obtained from National Centre for Cell Science (NCCS), Pune, India.

Techniques: Expressing

Journal: Journal of Natural Science, Biology, and Medicine

Article Title: Influence of limonin on Wnt signalling molecule in HepG2 cell lines

doi: 10.4103/0976-9668.107276

Figure Lengend Snippet: Differential mRNA expression of cyclin D1 in HepG2 cells

Article Snippet: Human liver-derived hepatoma G2 (HepG2) cells was obtained from National Centre for Cell Science (NCCS), Pune, India.

Techniques: Expressing

Journal: Journal of Natural Science, Biology, and Medicine

Article Title: Influence of limonin on Wnt signalling molecule in HepG2 cell lines

doi: 10.4103/0976-9668.107276

Figure Lengend Snippet: Western blotting analysis of p53 and Bcl2 protein expression in control and limonin treated HepG2 cells

Article Snippet: Human liver-derived hepatoma G2 (HepG2) cells was obtained from National Centre for Cell Science (NCCS), Pune, India.

Techniques: Western Blot, Expressing

Journal: Journal of Natural Science, Biology, and Medicine

Article Title: Influence of limonin on Wnt signalling molecule in HepG2 cell lines

doi: 10.4103/0976-9668.107276

Figure Lengend Snippet: Western blotting analysis of Bax and caspase-9 protein expression in control and limonin treated HepG2 cells

Article Snippet: Human liver-derived hepatoma G2 (HepG2) cells was obtained from National Centre for Cell Science (NCCS), Pune, India.

Techniques: Western Blot, Expressing

Journal: Journal of Natural Science, Biology, and Medicine

Article Title: Influence of limonin on Wnt signalling molecule in HepG2 cell lines

doi: 10.4103/0976-9668.107276

Figure Lengend Snippet: Western blotting analysis of Caspase-9 and Cyclin D1protein expression in control and limonin treated HepG2 cells

Article Snippet: Human liver-derived hepatoma G2 (HepG2) cells was obtained from National Centre for Cell Science (NCCS), Pune, India.

Techniques: Western Blot, Expressing